Bibframe Work

Title

Monoclonal antibodies

Type

Text

Monograph

Contribution

Ossipow, Vincent (editor)

Fischer, Nicolas (editor)

Springer Science+Business Media (copyright holder)

Subject

Monoclonal antibodies--Laboratory manuals (LCSH)

Antibodies, Monoclonal--Laboratory Manuals (MESH)

Genre Form

Laboratory Manuals (MESH)

Language

English (eng)

Illustrative Content

illustrations

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Classification

LCC: RB46.5 .M66 2014 (Assigner: dlc) (Status: used by assigner)

DDC: 616.07/980721 full (Source: 23)

Supplementary Content

bibliography (bibliography)

index (index)

Content

text (txt)

Summary

Monoclonal Antibodies: Methods and Protocols, Second Edition expands upon the previous edition with current, detailed modern approaches to isolate and characterize monoclonal antibodies against carefully selected epitopes. This edition includes new chapters covering the key steps to generate high quality monoclonals via different methods, from antigen generation to epitope mapping and quality control of the purified IgG. Chapters are divided into four parts corresponding to four distinct objectives. Part I covers monoclonal antibody generation, Part II deals with monoclonal antibody expression and purification, Part III presents methods for monoclonal antibody characterization and modification, and Part IV describes selected applications of monoclonal antibodies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Monoclonal Antibodies: Methods and Protocols, Second Edition provides crucial initial steps of monoclonal antibody generation and characterization with state-of-the art protocols.-- Source other than Library of Congress

Table Of Contents

Antigen production for monoclonal antibody generation / Giovanni Magistrelli and Pauline Malinge

Method for the generation of antibodies specific for site and posttranslational modifications / Hidemasa Goto and Masaki Inagaki

Immunization, hybridoma generation, and selection for monoclonal antibody production / Hyung-Yong Kim, Alexander Stojadinovic, and Mina J. Izadjoo

Hybridoma technology for the generation of rodent mAbs via classical fusion / Efthalia Chronopoulou [and three others]

Generation of rabbit monoclonal antibodies / Pi-Chen Yam and Katherine L. Knight

Screening hybridomas for cell surface antigens by high-throughput homogeneous assay and flow cytometry / Alejandro Uribe-Benninghoff [and four others]

Screening and subcloning of high producer transfectomas using semisolid media and automated colony picker / Suba Dharshanan and Cheah Swee Hung

Design and generation of synthetic antibody libraries for phage display / Gang Chen and Sachdev S. Sidhu

Selection and screening using antibody phage display libraries / Patrick Koenig and Germaine Fuh

Yeast surface display for antibody isolation : library construction, library screening, and affinity maturation / James A.Van Deventer and Karl Dane Wittrup

Human B cell immortalization for monoclonal antibody production / Joyce Hui-Yuen, Siva Koganti, and Sumita Bhaduri-McIntosh

Using next-generation sequencing for discovery of high- frequency monoclonal antibodies in the variable gene repertoires from immunized mice / Ulrike Haessler and Sai T. Reddy

Cloning, reformatting, and small-scale expression of monoclonal antibody isolated from mouse, rat, or hamster hybridoma / Jeremy Loyau and François Rousseau

Cloning of recombinant monoclonal antibodies from hybridomas in a single mammalian expression plasmid / Nicole Müller-Sienerth [and three others]

Monoclonal antibody purification by ceramic hydroxyapatite chromatography / Larry J. Cummings, Russell G. Frost, and Mark A. Snyder

Rapid purification of monoclonal antibodies using magnetic microspheres / Pauline Malinge and Giovanni Magistrelli

Generation of cell lines for monoclonal antibody production / Krista Alvin and Jianxin Ye

Expression and purification of recombinant antibody formats and antibody fusion proteins / Martin Siegemund [and four others]

Purification of antibodies and antibody fragments using CaptureSelect [trademark] affinity resins / Pim Hermans, Hendrik Adams, and Frank Detmers

Reformatting of scFv antibodies into the scFv-Fc format and their downstream purification / Emil Bujak [and three others]

Antibody V and C domain sequence, structure, and interaction analysis with special reference to IMGT® / Eltaf Alamyar [and three others]

Measuring antibody affinities as well as the active concentration of antigens present on a cell surface / Palaniswami Rathanaswami

Determination of antibody structures / Robyn L. Stanfield

Affinity maturation of monoclonal antibodies by multi-site- directed mutagenesis / Hyung-Yong Kim, Alexander Stojadinovic, and Mina J. Izadjoo

Epitope mapping with membrane-bound synthetic overlapping peptides / Terumi Midoro-Horiuti and Randall M. Goldblum

Epitope mapping by epitope excision, hydrogen/deuterium exchange, and peptide-panning techniques combined with in silico analysis/ Nicola Clementi [and five others]

Fine epitope mapping based on phage display and extensive mutagenesis of the target antigen / Gertrudis Rojas

Epitope mapping with random phage display library / Terumi Midoro-Horiuti and Randall M. Goldblum

Epitope mapping of monoclonal and polyclonal antibodies using bacterial cell surface display / Anna-Luisa Volk, Francis Jingxin Hu, and Johan Rockberg

Ion exchange-high-performance liquid chromatography (IEX-HPLC) / Marie Corbier, Delphine Schrag, and Sylvain Raimondi

Size exclusion-high-performance liquid chromatography (SEC-HPLC) / Delphine Schrag, Marie Corbier, and Sylvain Raimondi

N-glycosylation characterization by liquid chromatography with mass spectrometry / Song Klapoetke

Fc engineering of antibodies and antibody derivatives by primary sequence alteration and their functional characterization / Stefanie Derer [and six others]

Labeling and use of monoclonal antibodies in immunofluorescence : protocols for cytoskeletal and nuclear antigens / Christoph R. Bauer

Generation and use of antibody fragments for structural studies of proteins refractory to crystallization / Stephen J. Stahl, Norman R. Watts, and Paul T. Wingfield

Antibody array generation and use / Carl A.K. Borrebaeck and Christer Wingren

http://www.loc.gov/catdir/enhancements/fy1410/2013958232-t.html

Authorized Access Point

Monoclonal antibodies

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Status: new

Date: 2013-12-26

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Admin Metadata

Status: changed

Date: 2016-10-26T10:12:15

Description Modifier: United States, Library of Congress